Item no. |
ABC-AP7307a-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, IF |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CA2, CAC, CA-II, Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II |
Available |
|
Primary Accession |
P00918 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IF |
Bio Background |
CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. |
Bio References |
Sultana, R., et al. Antioxid. Redox Signal. 12(3):327-336(2010)Fisher, S.Z., et al. Biochemistry 49(3):415-421(2010)Avvaru, B.S., et al. Biochemistry 49(2):249-251(2010)Maupin, C.M., et al. Biochemistry 48(33):7996-8005(2009)Avvaru, B.S., et al. Biochemistry 48(31):7365-7372(2009) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB18368 |
Gene ID |
760 |
Gene Name |
CA2 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
Fluorescent confocal image of Hela cell stained with CA2 Antibody (N-term)(Cat#AP7307a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly. |
Type image 1 |
IF |
Dilution image 1 |
1:10~50 |
Legend image 2 |
Western blot analysis of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody (N-term) (Cat. #AP7307a). AP7307a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
CA2 Antibody (N-term) (Cat.# AP7307a) western blot analysis in MCF-7 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the CA2 antibody detected the CA2 protein (arrow). |
Type image 3 |
WB |
Dilution image 3 |
1:1000 |
Isotype |
Rabbit Ig |
Calculated MW |
29246 |
Antigen Region |
54-83 |
Antigen Source |
HUMAN |
Target/Specificity |
This CA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CA2. |
NCBI Accession |
NP_000058.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.