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CA2 Antibody (N-term)

CA2 Antibody (N-term)

Item no.	ABC-AP7307a-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, IF
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	CA2, CAC, CA-II, Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II
Available
Primary Accession	P00918
Antigen Type	Synthetic Peptide
Application	WB, IF
Bio Background	CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis.
Bio References	Sultana, R., et al. Antioxid. Redox Signal. 12(3):327-336(2010) Fisher, S.Z., et al. Biochemistry 49(3):415-421(2010) Avvaru, B.S., et al. Biochemistry 49(2):249-251(2010) Maupin, C.M., et al. Biochemistry 48(33):7996-8005(2009) Avvaru, B.S., et al. Biochemistry 48(31):7365-7372(2009)
Clonality	Polyclonal
Clone/Animal Names	RB18368
Gene ID	760
Gene Name	CA2
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Fluorescent confocal image of Hela cell stained with CA2 Antibody (N-term)(Cat#AP7307a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly.
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody (N-term) (Cat. #AP7307a). AP7307a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	CA2 Antibody (N-term) (Cat.# AP7307a) western blot analysis in MCF-7 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the CA2 antibody detected the CA2 protein (arrow).
Type image 3	WB
Dilution image 3	1:1000
Isotype	Rabbit Ig
Calculated MW	29246
Antigen Region	54-83
Antigen Source	HUMAN
Target/Specificity	This CA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CA2.
NCBI Accession	NP_000058.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 80 ul

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Delivery expected until 11/8/2024

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