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PIST Antibody (C-term)

PIST Antibody (C-term)

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, IHC-P
Amount	80 ul
Host	Rabbit
Item no.	ABC-AP7262b-ev
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q9HD26
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
Bio References	Li, X., Protein Sci. 15 (9), 2149-2158 (2006) Ito, H., Biochem. J. 397 (3), 389-398 (2006) Wente, W., J. Biol. Chem. 280 (37), 32419-32425 (2005)
Clonality	Polyclonal
Clone/Animal Names	RB13073
Gene ID	57120
Gene Name	GOPC
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Western blot analysis of PIST Antibody (C-term) (Cat.#AP7262b) in Ramos cell line lysates (35ug/lane). PIST (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PIST antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:10~50
Other Accession	Q8BH60
Isotype	Rabbit Ig
Calculated MW	50520
Antigen Region	361-390
Antigen Source	HUMAN
Target/Specificity	This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-390 amino acids from the C-terminal region of human PIST.
NCBI Accession	NP_001017408.1; NP_065132.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-AP7262b-ev-datasheet.pdf

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Amount: 80 ul

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Delivery expected until 9/6/2024

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