Item no. |
ABC-AP6906C-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
COMP, Cartilage oligomeric matrix protein, Thrombospondin-5, TSP5 |
Available |
|
Primary Accession |
P49747 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB, FC |
Bio Background |
COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. |
Bio References |
Kim, H.J., et.al., Eur. J. Appl. Physiol. 105 (5), 765-770 (2009) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB21199 |
Gene ID |
1311 |
Gene Name |
COMP |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-COMP Antibody (Center) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: ATDC5 whole cell lysateLane 3: HUVEC whole cell lysateLane 4: Jurkat whole cell lysateLane 5: MG-63 whole cell lysateLane 6: U-2OS whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83, 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Legend image 2 |
Anti-COMP Antibody (Center) at 1:2000 dilution + Rat skeletal muslce lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:2000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with COMP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:10~50 |
Legend image 4 |
Overlay histogram showing HepG2 cells stained with AP6906C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6906C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Type image 4 |
FC |
Dilution image 4 |
1:25 |
Legend image 5 |
Overlay histogram showing HepG2 cells stained with AP6906C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6906C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Type image 5 |
FC |
Dilution image 5 |
1:25 |
Isotype |
Rabbit Ig |
Calculated MW |
82860 |
Antigen Region |
314-343 |
Antigen Source |
HUMAN |
Target/Specificity |
This COMP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the Central region of human COMP. |
NCBI Accession |
NP_000086.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.