Item no. |
ABC-AP6753C-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, IF, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
Smad7, SMAD7, MADH7, MADH8, Mothers against decapentaplegic homolog 7, MAD homolog 7, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 8, Mothers against DPP homolog 8, SMAD family member 7, SMAD 7, hSMAD7 |
Available |
|
Primary Accession |
O15105 |
Antigen Type |
Synthetic Peptide |
Application |
IF, IHC-P, WB |
Bio Background |
Smad7 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta super family. |
Bio References |
Hirata, H., et.al., Cancer 115 (19), 4488-4503 (2009) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB18598 |
Gene ID |
4092 |
Gene Name |
SMAD7 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
"Fluorescent confocal image of A431 cell stained with Smad7 Antibody (Center)(Cat#AP6753c). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Smad7 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).Smad7 immunoreactivity is localized to cytoplasm significantly." |
Type image 1 |
IF |
Dilution image 1 |
1:10~50 |
Legend image 2 |
Western blot analysis of Smad7 Antibody (Center) (Cat. #AP6753c) in ZR-75-1 cell line lysates (35ug/lane). Smad7 (arrow) was detected using the purified Pab. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Smad7 Antibody (Center) (Cat. #AP6753c) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Smad7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:10~50 |
Other Accession |
O88406 |
Predicted |
Rat |
Isotype |
Rabbit Ig |
Calculated MW |
46426 |
Antigen Region |
195-224 |
Antigen Source |
HUMAN |
Target/Specificity |
This Smad7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids from the Central region of human Smad7. |
NCBI Accession |
NP_001177750.1; NP_001177751.1; NP_001177752.1; NP_005895.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.