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PDIA6 Antibody (Center K159)

PDIA6 Antibody (Center K159)

Item no.	ABC-AP6662b-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PDIA6, P5, ERP5, TXNDC7, Protein disulfide-isomerase A6, ERp5, Endoplasmic reticulum protein 5, ER protein 5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7
Available
Primary Accession	Q15084
Antigen Type	Synthetic Peptide
Application	IF, IHC-P, WB, FC
Bio Background	Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.
Bio References	Hayano, T., Gene 164 (2), 377-378 (1995)
Clonality	Polyclonal
Clone/Animal Names	RB20199
Gene ID	10130
Gene Name	PDIA6
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Rat
Legend image 1	Fluorescent image of HepG2 cells stained with XAF1 PDIA6 Antibody (Center K159)(Cat#AP6662b). AP6662b was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Type image 1	IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of PDIA6 antibody (Center K159) (Cat.# AP6662b) in Y79 cell line lysates (35ug/lane). PDIA6 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of PDIA6 antibody (Center K159) (Cat.# AP6662b) in mouse stomach tissue lysates (35ug/lane). PDIA6 (arrow) was detected using the purified Pab.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Western blot analysis of lysates from K562, HepG2, HT-1080, rat C6 cell line and human liver tissue lysate (from left to right), using PDIA6 Antibody (Center K159)(Cat. #AP6662b). AP6662b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center K159), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 5	IHC-P
Dilution image 5	1:50~100
Legend image 6	Immunohistochemical analysis of paraffin-embedded H. liver section using PDIA6 Antibody (Center K159)(Cat#AP6662b). AP6662b was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 6	IHC-P
Dilution image 6	1:100
Legend image 7	Immunohistochemical analysis of paraffin-embedded H. brain section using PDIA6 Antibody (Center K159)(Cat#AP6662b). AP6662b was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 7	IHC-P
Dilution image 7	1:100
Isotype	Rabbit Ig
Calculated MW	48121
Antigen Region	144-172
Antigen Source	HUMAN
Target/Specificity	This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human PDIA6.
NCBI Accession	NP_005733.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 80 ul

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Delivery expected until 10/4/2024

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