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CD14 Antibody (N-term)

CD14 Antibody (N-term)

Item no.	ABC-AP6294A
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	CD14, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, membrane-bound form, urinary form
Available
Primary Accession	P08571
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, WB, IF, IHC-P-Leica
Bio Background	CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
Bio References	Donati, M., J. Periodontol. 79 (3), 517-524 (2008) Yuan, F.F., Immunol. Cell Biol. 86 (3), 268-270 (2008) Setoguchi, M., Biochim. Biophys. Acta 1008 (2), 213-222 (1989) Goyert, S.M., Science 239 (4839), 497-500 (1988)
Clonality	Polyclonal
Clone/Animal Names	RB14105
Gene ID	929
Gene Name	CD14
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling CD14 with AP6294A at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG (OH191631) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and membrance staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	All lanes : Anti-CD14 Antibody (N-term) at 1:2000 dilution Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lane 3: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	All lanes : Anti-CD14 Antibody (N-term) at 1:1000 dilution Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	All lanes : Anti-CD14 Antibody (N-term) at 1:1000 dilution Lane 1: THP-1 whole cell lysate Lane 2: Human lung tissue lysate Lane 3: Human liver tissue lysate Lane 4: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	All lanes : Anti-CD14 Antibody (N-term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 5	WB
Dilution image 5	1:2000
Legend image 6	Immunohistochemical analysis of paraffin-embedded human liver tissue using AP6294A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 6	IHC-P-Leica
Dilution image 6	1:1000
Legend image 7	Overlay histogram showing Jurkat cells stained with AP6294A(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 7	FC
Dilution image 7	1:25
Isotype	Rabbit IgG
Calculated MW	40076
Antigen Region	54-83
Antigen Source	HUMAN
Target/Specificity	This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14.
NCBI Accession	NP_000582.1; NP_001035110.1; NP_001167575.1; NP_001167576.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 9/6/2024

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