Item no. |
ABC-AP6166a |
Manufacturer |
Abcepta
|
Amount |
400 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
MAGEA4, MAGE4, Melanoma-associated antigen 4, MAGE-4 antigen, MAGE-41 antigen, MAGE-X2 antigen, CT14, Cancer/testis antigen 14 |
Available |
|
Primary Accession |
P43358 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
MAGEA4 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in somehereditary disorders, such as dyskeratosis congenita. |
Bio References |
Nagao, T., et al., J. Biol. Chem. 278(12):10668-10674 (2003).Resnick, M.B., et al., Int. J. Cancer 101(2):190-195 (2002).Imai, Y., et al., Gene 160(2):287-290 (1995).Rogner, U.C., et al., Genomics 29(3):725-731 (1995).De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB2196 |
Gene ID |
4103 |
Gene Name |
MAGEA4 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
The anti-MAGE-A4 Pab (Cat. #AP6166a) is used in Western blot to detect MAGE-A4 in A375 cell lysate. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Immunostaining of paraformldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody Cat# AP6166a (1:50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X. Data courtesy of Dr. Mi Hou, Karolinska?Institutet and University Hospital, Sweden. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:50~100 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody (N-term) (Cat.#AP6166a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Isotype |
Rabbit Ig |
Calculated MW |
34899 |
Antigen Region |
9-38 |
Antigen Source |
HUMAN |
Target/Specificity |
This MAGEA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human MAGEA4. |
NCBI Accession |
NP_001011548.1; NP_001011549.1; NP_001011550.1; NP_002353.3 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.