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FMNL3 Polyclonal Antibody

FMNL3 Polyclonal Antibody

Item no.	ABC-AP56152
Manufacturer	Abcepta
Amount	100 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, IF, ICC, IHC-P, IHC-F
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	FHOD3, FMNL3, WBP3, Formin-like protein 3, Formin homology 2 domain-containing protein 3, WW domain-binding protein 3, WBP-3, FRL2, KIAA2014
Available
Primary Accession	Q8IVF7
Application	WB, IHC-P, IHC-F, IF, ICC
Clonality	Polyclonal
Gene ID	91010
Gene Name	FMNL3
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	Rat, Dog, Cow
Legend image 1	Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMNL3) Polyclonal Antibody, Unconjugated (bs-16151R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Type image 1	IHC-P
Legend image 2	Blank control: Molt4. Primary Antibody (green line): Rabbit Anti- FMNL3/FITC Conjugated antibody (bs-16151R-FITC) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20, 000 events was performed.
Type image 2	FC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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