Item no. |
ABC-AP54185 |
Manufacturer |
Abcepta
|
Amount |
100 ul |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IF, ICC, IHC-P, IHC-F |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
Nociceptin receptor, KOR-3, ORGC, Oprl, K3 opiate receptor, Kappa-type 3 opioid receptor, Orphanin FQ receptor, Oprl1, Oor |
Available |
|
Primary Accession |
P35377 |
Application |
WB, IHC-P, IHC-F, IF, ICC |
Clonality |
Polyclonal |
Gene ID |
18389 |
Gene Name |
Oprl1 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
Rat, Pig, Dog, Cow |
Legend image 1 |
Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Nociceptin receptor (bs-0181R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 52 kD |
Type image 1 |
WB |
Legend image 2 |
Sample: Heart(Rat) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-Nociceptin receptor (bs-0181R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 40kD Observed band size : 40kD |
Type image 2 |
WB |
Legend image 3 |
免疫荧光Immunofluorescence 组织切片: Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(bs-0181R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. |
Type image 3 |
IF |
Legend image 4 |
免疫组化(IHC)Immunohistochemistry Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(bs-0181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
Type image 4 |
IHC-P |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.