Item no. |
ABC-AP5009a-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
AP19, AP1S1, CLAPS1, AP-1 complex subunit sigma-1A, HA1 19 kDa subunit, Clathrin assembly protein complex 1 sigma-1A small chain, Clathrin coat assembly protein AP19, Golgi adaptor HA1/AP1 adaptin sigma-1A subunit, Sigma 1a subunit of AP-1 clathrin, Sigma-adaptin 1A, Sigma1A-adaptin, Adaptor protein complex AP-1 subunit sigma-1A, Adaptor-related protein complex 1 subunit sigma-1A |
Available |
|
Primary Accession |
P61966 |
Antigen Type |
Synthetic Peptide |
Application |
FC, IHC-P, WB |
Bio Background |
AP1S1 is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle. |
Bio References |
Montpetit, A., et al. PLoS Genet. 4 (12), E1000296 (2008) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) Leong, W.F., et al. Cell. Microbiol. 8(4):565-580(2006) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB25813 |
Gene ID |
1174 |
Gene Name |
AP1S1 |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of AP1S1 Antibody (N-Term)(Cat. #AP5009a) in MDA-MB231 cell line lysates (35ug/lane).AP1S1 (arrow) was detected using the purified Pab. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
AP1S1 Antibody (N-Term) (Cat. #AP5009a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AP1S1 Antibody (N-Term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:50~100 |
Legend image 3 |
AP1S1 Antibody (N-Term) (Cat. #AP5009a) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 3 |
FC |
Dilution image 3 |
1:10~50 |
Other Accession |
P61967; Q1JQ98 |
Predicted |
B, M |
Isotype |
Rabbit Ig |
Calculated MW |
18733 |
Antigen Region |
5-34 |
Antigen Source |
HUMAN |
Target/Specificity |
This AP1S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-34 amino acids from the N-terminal region of human AP1S1. |
NCBI Accession |
NP_001274.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.