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phospho-Sox2(S246) Antibody

phospho-Sox2(S246) Antibody

Item no.	ABC-AP3750a-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	IF, Dot
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SOX2, Transcription factor SOX-2
Available
Primary Accession	P48431
Antigen Type	Synthetic Peptide
Application	DB, IF
Bio Background	This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Bio References	References for protein: 1.Gen, Y., et al. Cancer Genet. Cytogenet. 202(2):82-93(2010) 2.Ji, J., et al. Hum. Pathol. 41(10):1438-1447(2010) 3.Fang, X., et al. OMICS 14(4):369-384(2010) 4.Sholl, L.M., et al. Am. J. Surg. Pathol. 34(8):1193-1198(2010) 5.Zhang, D., et al. BMC Med. Genet. 11, 116 (2010) References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
Clonality	Polyclonal
Clone/Animal Names	RB26203
Gene ID	6657
Gene Name	SOX2
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Fluorescent confocal image of SY5Y cells stained with phospho-Sox2- S246 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3750a phospho-Sox2- S246 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus.
Type image 1	IF
Dilution image 1	1:200
Legend image 2	Dot blot analysis of anti-phospho-Sox2-pS246 Phospho-specific Pab (Cat. #AP3750a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Type image 2	DB
Dilution image 2	1:500
Other Accession	P48432; NP_003097.1; P54231
Predicted	M, Sh
Isotype	Rabbit Ig
Calculated MW	34310
Antigen Source	HUMAN
Target/Specificity	This Sox2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S246 of human Sox2.
NCBI Accession	NP_003097.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 80 ul

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