« Back
Home /
Phospho-MeCP2(S423) Antibody

Phospho-MeCP2(S423) Antibody

Item no.	ABC-AP3693a
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	MECP2, Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2
Available
Primary Accession	P51608
Antigen Type	Synthetic Peptide
Application	IHC-P-Leica, WB
Bio Background	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Bio References	Mnatzakanian, G.N., et al., Nat. Genet. 36(4):339-341 (2004). Laccone, F., et al., Hum. Mutat. 23(3):234-244 (2004). Suzuki, M., et al., Oncogene 22(54):8688-8698 (2003). Balmer, D., et al., J. Mol. Med. 81(1):61-68 (2003). Hagberg, B., et al., Eur. J. Paediatr. Neurol. 7(6):417-421 (2003).
Clonality	Polyclonal
Clone/Animal Names	RB29261
Gene ID	4204
Gene Name	MECP2
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, Rat
Legend image 1	Western blot analysis of lysates from Neuro-2a cell line, untreated or treated with calf intestinal alkaline phosphatase(CIP), using Phospho-MeCP2(S423) Antibody(upper) or Beta-actin (lower).
Type image 1	WB
Dilution image 1	1:500
Legend image 2	Western blot analysis of lysates from Jurkat cell line, untreated or treated with calf intestinal alkaline phosphatase(CIP), using Phospho-MeCP2(S423) Antibody(upper) or Beta-actin (lower).
Type image 2	WB
Dilution image 2	1:500
Legend image 3	Western blot analysis of lysates from C6 cell line, untreated or treated with calf intestinal alkaline phosphatase(CIP), using Phospho-MeCP2(S423) Antibody(upper) or Beta-actin (lower).
Type image 3	WB
Dilution image 3	1:500
Legend image 4	Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP3693a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 4	IHC-P-Leica
Dilution image 4	1:500
Other Accession	Q9Z2D6; Q95LG8
Predicted	Pr, M
Isotype	Rabbit IgG
Calculated MW	52441
Antigen Source	HUMAN
Target/Specificity	This MeCP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S423 of human MeCP2.
NCBI Accession	NP_001104262.1; NP_004983.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-AP3693a-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 400 ul

available

Delivery expected until 9/6/2024

Express Delivery: Save 1 days

Compare

Add to wishlist

Recommend by mail