Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, FC, IHC-P |
Amount |
80 ul |
Host |
Rabbit |
Item no. |
ABC-AP2926a-ev |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
P30048 |
Antigen Type |
Synthetic Peptide |
Application |
WB, FC, IHC-P |
Bio Background |
PRDX3 is a protein with antioxidant function and is localized in the mitochondrion. This protein is involved in redox regulation of the cell and Protects radical-sensitive enzymes from oxidative damage by a radical-generating system.It Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. |
Bio References |
Cox, A.G., et.al., Biochemistry 48 (27), 6495-6501 (2009) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB20889 |
Gene ID |
10935 |
Gene Name |
PRDX3 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of PRDX3 Antibody (N-term) (Cat. #AP2926a) in NCI-H460, MDA-MB231, HepG2 cell line lysates (35ug/lane). PRDX3 (arrow) was detected using the purified Pab.(2ug/ml) |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of PRDX3 (arrow) using rabbit polyclonal PRDX3 Antibody (N-term) (Cat. #AP2926a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX3 gene. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRDX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Legend image 4 |
Flow cytometric analysis of MDA-MB468 cells using PRDX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 4 |
FC |
Dilution image 4 |
1:10~50 |
Isotype |
Rabbit Ig |
Calculated MW |
27693 |
Antigen Region |
66-94 |
Antigen Source |
HUMAN |
Target/Specificity |
This PRDX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PRDX3. |
NCBI Accession |
NP_006784.1; NP_054817.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.