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MCAM Antibody (Center)

MCAM Antibody (Center)

Item no.	ABC-AP2767C-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	MCAM, CD146, MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen
Available
Primary Accession	P43121
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, WB
Bio Background	MCAM plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. It could be an adhesion molecule active in neural crest cells during embryonic development. It acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
Bio References	Fritzsche, F.R., Pathology 40 (5), 457-464 (2008) Malyszko, J., Clin. Appl. Thromb. Hemost. 14 (3), 338-345 (2008) Guezguez, B., J. Immunol. 179 (10), 6673-6685 (2007)
Clonality	Polyclonal
Clone/Animal Names	RB20606
Gene ID	4162
Gene Name	MCAM
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-MCAM Antibody (Center) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human skin reacted with MCAM Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:50~100
Legend image 3	Flow cytometric analysis of hela cells using MCAM Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Other Accession	NP_006491
Isotype	Rabbit Ig
Calculated MW	71607
Antigen Region	161-189
Antigen Source	HUMAN
Target/Specificity	This MCAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-189 amino acids from the Central region of human MCAM.
NCBI Accession	NP_006491.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 80 ul

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Delivery expected until 10/4/2024

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