Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, IHC-P |
Amount |
80 ul |
Host |
Rabbit |
Item no. |
ABC-AP2729b-ev |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
O75829 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P |
Bio Background |
LECT1 a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This protein is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. |
Bio References |
Aoyama, T., Biochem. Biophys. Res. Commun. 365 (1), 124-130 (2008)Yoshioka, M., Nat. Med. 12 (10), 1151-1159 (2006)Aoyama, T., J. Biol. Chem. 279 (27), 28789-28797 (2004) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB15073 |
Gene ID |
11061 |
Gene Name |
|
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of anti-LECT1 Antibody (C-term) (Cat.#AP2729b) in NCI-H460 cell line lysates (35ug/lane).LECT1(arrow) was detected using the purified Pab. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of LECT1 (arrow) using rabbit polyclonal LECT1 Antibody (C-term) (Cat.#AP2729b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LECT1 gene. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with LECT1 antibody (C-term) (Cat.#Ap2729b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:10~50 |
Other Accession |
O70367; O77770; Q9Z1F6; P17404 |
Predicted |
B, M, Rb, Rat |
Isotype |
Rabbit Ig |
Calculated MW |
37102 |
Antigen Region |
253-281 |
Antigen Source |
HUMAN |
Target/Specificity |
This LECT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the C-terminal region of human LECT1. |
NCBI Accession |
NP_001011705.1; NP_008946.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.