p53 Antibody (Sumoylation Site Specific)

Synthetic Peptide

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.

Polyclonal

7157

Purified Rabbit Polyclonal Antibody (Pab)

TP53 Antibody (C-term) (Cat. #AP2505b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TP53 antibody detected the TP53 protein (arrow).

1:1000

WB

Immunohistochemical analysis of paraffin-embedded H. esophagus section using p53 Antibody (Sumoylation Site Specific)(Cat#AP2505b). AP2505b was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

1:100

43653

HUMAN

NP_000537.3; NP_001119584.1; NP_001119585.1; NP_001119586.1; NP_001119587.1; NP_001119588.1; NP_001119589.1; NP_001119590.1; NP_001263624.1; NP_001263625.1; NP_001263626.1; NP_001263627.1; NP_001263628.1; NP_00126