Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC, IF |
Amount |
200 ul |
Host |
Rabbit |
Item no. |
ABC-AP22336a |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
P28676 |
Antigen Type |
Synthesized Peptide |
Application |
WB, FC, IF |
Bio Background |
Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions. |
Bio References |
Boyhan A., et al.J. Biol. Chem. 267:2928-2933(1992).Ota T., et al.Nat. Genet. 36:40-45(2004).Totoki Y., et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.Hillier L.W., et al.Nature 434:724-731(2005).Mural R.J., et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. |
Clonality |
polyclonal |
Clone/Animal Names |
RB57950 |
Gene ID |
25801 |
Gene Name |
GCA |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling GCA with AP22336a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue). |
Type image 1 |
IF |
Dilution image 1 |
1:25 |
Legend image 2 |
All lanes : Anti-GCA Antibody (N-Term) at 1:2000 dilutionLane 1: Human cerebellum lysateLane 2: Human spleen lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:2000 |
Legend image 3 |
Overlay histogram showing U-2 OS cells stained with AP22336a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22336a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Type image 3 |
FC |
Dilution image 3 |
1:25 |
Isotype |
Rabbit Ig |
Calculated MW |
24010 |
Antigen Source |
HUMAN |
Target/Specificity |
This GCA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-53 amino acids from the human region of human GCA. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.