CKS2 Antibody (N-Term)

P33552

WB, FC

Richardson H.E., et al.Genes Dev. 4:1332-1344(1990).
Kalnine N., et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L., et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J., et al.Nature 429:369-374(2004).
Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

RB30893

CKS2

H

WB

Overlay histogram showing HeLa cells stained with AP22318a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22318a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

B, M

9860

This CKS2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 6-40 amino acids from the human region of human CKS2.