Item no. |
ABC-AP22301a-ev |
Manufacturer |
Abcepta
|
Amount |
50 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
EP300, P300, Histone acetyltransferase p300, E1A-associated protein p300, p300 HAT, 2.3.1.48 |
Available |
|
Primary Accession |
Q09472 |
Antigen Type |
Synthesized Peptide |
Application |
WB |
Bio Background |
Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed: 10733570, PubMed: 11430825, PubMed: 11701890, PubMed: 12402037, PubMed: 12586840, PubMed: 12929931, PubMed: 14645221, PubMed: 15186775, PubMed: 15890677, PubMed: 16617102, PubMed: 16762839, PubMed: 18722353, PubMed: 18995842, PubMed: 23415232, PubMed: 23911289, PubMed: 23934153, PubMed: 8945521). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (By similarity). |
Bio References |
Eckner R., et al.Genes Dev. 8:869-884(1994).Dunham I., et al.Nature 402:489-495(1999).Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.Chaffanet M., et al.Genes Chromosomes Cancer 28:138-144(2000).Lundblad J.R., et al.Nature 374:85-88(1995). |
Clonality |
polyclonal |
Clone/Animal Names |
RB58228 |
Gene ID |
2033 |
Gene Name |
EP300 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Anti-EP300(S1834) at 1:2000 dilution + U-2 OS whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 264 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Isotype |
Rabbit Ig |
Calculated MW |
264161 |
Antigen Source |
HUMAN |
Target/Specificity |
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1807-1841 amino acids from human. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.