Item no. |
ABC-AP22275a |
Manufacturer |
Abcepta
|
Amount |
200 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, IF |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
FADS2, Fatty acid desaturase 2, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, 1.14.19.- |
Available |
|
Primary Accession |
O95864 |
Antigen Type |
Recombinant Protein |
Application |
WB, IF |
Bio Background |
Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma- linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum. |
Bio References |
Cho H.P., et al.J. Biol. Chem. 274:471-477(1999).Marquardt A., et al.Genomics 66:175-183(2000).Zhang J.S.S., et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.Ota T., et al.Nat. Genet. 36:40-45(2004).Otsuki T., et al.DNA Res. 12:117-126(2005). |
Clonality |
polyclonal |
Clone/Animal Names |
RB56816 |
Gene ID |
9415 |
Gene Name |
|
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling FADS2 with AP22275a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG (OH191631) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue). |
Type image 1 |
IF |
Dilution image 1 |
1:25 |
Legend image 2 |
Anti-FADS2 Antibody at 1:2000 dilution + Human liver lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:2000 |
Isotype |
Rabbit Ig |
Calculated MW |
52259 |
Antigen Source |
HUMAN |
Target/Specificity |
This FADS2 antibody is generated from a rabbit immunized with a recombinant protein of human FADS2. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.