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SLC2A8 Antibody (Center)

SLC2A8 Antibody (Center)

Item no.	ABC-AP22138c
Manufacturer	Abcepta
Amount	200 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SLC2A8, Solute carrier family 2, GLUT8, GLUTX1, facilitated glucose transporter member 8, Glucose transporter type 8, GLUT-8, Glucose transporter type X1
Available
Primary Accession	Q9NY64
Antigen Type	Synthesized Peptide
Application	WB, IHC-P, FC
Bio Background	Insulin-regulated facilitative glucose transporter. Binds cytochalasin B in a glucose-inhibitable manner. Seems to be a dual-specific sugar transporter as it is inhibitable by fructose (By similarity).
Bio References	Doege H., et al.J. Biol. Chem. 275:16275-16280(2000). Ibberson M.R., et al.J. Biol. Chem. 275:4607-4612(2000). Humphray S.J., et al.Nature 429:369-374(2004). Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Burkard T.R., et al.BMC Syst. Biol. 5:17-17(2011).
Clonality	polyclonal
Clone/Animal Names	RB55905
Gene ID	29988
Gene Name	SLC2A8 (HGNC:13812)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Rat
Legend image 1	All lanes : Anti-SLC2A8 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: human testis lysate Lane 4: Caco2 whole cell lysate Lane 5: mouse testis lysate Lane 6: rat testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	AP22138c staining SLC2A8 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 2	IHC-P
Dilution image 2	1:25
Legend image 3	Overlay histogram showing U-2 OS cells stained with AP22138c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22138c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 3	FC
Dilution image 3	1:25
Other Accession	P58354
Predicted	B
Isotype	Rabbit Ig
Calculated MW	50819
Antigen Source	HUMAN
Target/Specificity	This SLC2A8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 260-292 amino acids from the Central region of human SLC2A8.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200 ul

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Delivery expected until 9/6/2024