Item no. |
ABC-AP22077a-ev |
Manufacturer |
Abcepta
|
Amount |
50 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
RPS7, 40S ribosomal protein S7 |
Available |
|
Primary Accession |
P62081 |
Antigen Type |
Synthesized Peptide |
Application |
WB, FC |
Bio Background |
Required for rRNA maturation. |
Bio References |
Annilo T., et al.Gene 165:297-302(1995).Ota T., et al.Nat. Genet. 36:40-45(2004).Hillier L.W., et al.Nature 434:724-731(2005).Mural R.J., et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.Vladimirov S.N., et al.Eur. J. Biochem. 239:144-149(1996). |
Clonality |
polyclonal |
Clone/Animal Names |
RB55875 |
Gene ID |
6201 |
Gene Name |
RPS7 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
All lanes : Anti-RPS7 Antibody (N-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Jurkat whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Legend image 2 |
Overlay histogram showing Hela cells stained with AP22077a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22077a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Type image 2 |
FC |
Dilution image 2 |
1:25 |
Other Accession |
A6H769; P62084; Q5RT64; P62082; P62083; P50894; P02362 |
Predicted |
B, Zf, Rat, X |
Isotype |
Rabbit Ig |
Calculated MW |
22127 |
Antigen Source |
HUMAN |
Target/Specificity |
This RPS7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 7-40 amino acids from human RPS7. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.