Item no. |
ABC-AP21782a-ev |
Manufacturer |
Abcepta
|
Amount |
50 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
IRF3, IRF-3, Interferon regulatory factor 3 |
Available |
|
Primary Accession |
Q14653 |
Application |
WB |
Bio Background |
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages. |
Bio References |
Au W.W.-C., et al.Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995).Tabata Y., et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.Ota T., et al.Nat. Genet. 36:40-45(2004).Grimwood J., et al.Nature 428:529-535(2004).Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. |
Clonality |
polyclonal |
Clone/Animal Names |
RB53950 |
Gene ID |
3661 |
Gene Name |
IRF3 {ECO:0000303|PubMed:9803267, ECO:0000312|HGNC:HGNC:6118} |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-IRF3 Antibody (N-Term) at 1:2000 dilutionLane 1: Raji whole cell lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Isotype |
Rabbit Ig |
Calculated MW |
47219 |
Antigen Source |
HUMAN |
Target/Specificity |
This IRF3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from human IRF3. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.