Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC |
Amount |
80 ul |
Host |
Rabbit |
Item no. |
ABC-AP21048a-ev |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
P50225 |
Application |
WB, FC |
Bio Background |
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N- hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. |
Bio References |
Zhu X., et al.Biochem. Biophys. Res. Commun. 195:120-127(1993).Zhu X., et al.Biochem. Biophys. Res. Commun. 192:671-676(1993).Wilborn T.W., et al.Mol. Pharmacol. 43:70-77(1993).Yamazoe Y., et al.Chem. Biol. Interact. 92:107-117(1994).Hwang S.-R., et al.Biochem. Biophys. Res. Commun. 207:701-707(1995). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB51774 |
Gene ID |
6817 |
Gene Name |
SULT1A1 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of lysates from HepG2 cell line and human lung tissue (from left to right), using SULT1A1 Antibody (C-term)(Cat. #AP21048a). AP21048a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Overlay histogram showing HepG2 cells stained with AP21048a (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21048a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Type image 2 |
FC |
Dilution image 2 |
1:25 |
Other Accession |
P50226 |
Isotype |
Rabbit IgG |
Calculated MW |
34165 |
Antigen Source |
HUMAN |
Target/Specificity |
This SULT1A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 246-279 amino acids of human SULT1A1. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.