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(Mouse) Trim24 Antibody (C-term)

(Mouse) Trim24 Antibody (C-term)

Item no.	ABC-AP20896c-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IF, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Transcription intermediary factor 1-alpha, Tripartite motif-containing protein 24, E3 ubiquitin-protein ligase Trim24, 632-, TIF1-alpha, Trim24, Tif1, Tif1a
Available
Primary Accession	Q64127
Application	WB, IF, IHC-P
Bio Background	Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac) (By similarity). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis via its effects on p53/TP53 levels. Up-regulates ligand- dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, such as RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. Required for normal transition from proliferating neonatal hepatocytes to quiescent adult hepatocytes.
Bio References	le Douarin B., et al.EMBO J. 14:2020-2033(1995). le Douarin B., et al.EMBO J. 15:6701-6715(1996). Zhong S., et al.Nat. Genet. 23:287-295(1999). Seeler J.-S., et al.Mol. Cell. Biol. 21:3314-3324(2001). Torres-Padilla M.E., et al.J. Cell Biol. 174:329-338(2006).
Clonality	Polyclonal
Clone/Animal Names	RB51160
Gene ID	21848
Gene Name	Trim24
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	M
Legend image 1	Fluorescent image of Hela cells stained with (Mouse) Trim24 Antibody (C-term)(Cat#AP20896c). AP20896c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	Western blot analysis of lysates from mouse NIH/3T3 cell line, mouse spleen, mouse testis, mouse thymus tissue lysate (from left to right), using Trim24 Antibody (C-term)(Cat. #AP20896c). AP20896c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Anti-Trim24 Antibody (C-term)at 1:2000 dilution + NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 117 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:2000
Legend image 4	AP20896c staining (Mouse) Trim24 in mouse testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 4	IHC-P
Dilution image 4	1:25
Isotype	Rabbit Ig
Calculated MW	116657
Antigen Source	HUMAN
Target/Specificity	This Mouse Trim24 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 789-843 amino acids from the C-terminal region of Mouse Trim24.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 80 ul

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Delivery expected until 9/6/2024

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