Item no. |
ABC-AP20520b |
Manufacturer |
Abcepta
|
Amount |
400 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, IF, IHC-P |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
POLR2C, DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB33, RPB31, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C |
Available |
|
Primary Accession |
P19387 |
Antigen Type |
Synthetic Peptide |
Application |
IF, WB, IHC-P |
Bio Background |
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity). |
Bio References |
Pati U.K., et al. J. Biol. Chem. 265:8400-8403(1990).Dammann R., et al. Biochim. Biophys. Acta 1396:153-157(1998).Bruno T., et al. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.Loftus B.J., et al. Genomics 60:295-308(1999).Bienvenut W.V., et al. Submitted (MAR-2009) to UniProtKB. |
Clonality |
Polyclonal |
Gene ID |
5432 |
Gene Name |
|
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
Fluorescent image of Hela cells stained with POLR2C Antibody (C-term)(Cat#AP20520B). AP20520B was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red). |
Type image 1 |
IF |
Dilution image 1 |
1:25 |
Legend image 2 |
POLR2C Antibody (C-term) (Cat. #AP20520b) western blot analysis in Hela, U251 cell line and mouse skeletal muscle tissue lysates (35ug/lane).This demonstrates the POLR2C antibody detected the POLR2C protein (arrow). |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded H.brain, H.cerebellum tissue reacted with POLR2C Antibody (C-term) (Cat#AP20520b). |
Type image 3 |
IHC-P |
Dilution image 3 |
1:100 |
Other Accession |
P97760; Q3T0Q3 |
Predicted |
B |
Isotype |
Rabbit Ig |
Calculated MW |
31441 |
Antigen Region |
200-228 |
Antigen Source |
Human |
Target/Specificity |
This POLR2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the C-terminal region of human POLR2C. |
NCBI Accession |
NP_116558.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.