Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB |
Amount |
400 ul |
Host |
Rabbit |
Item no. |
ABC-AP20489a |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
Q13322 |
Antigen Type |
Synthetic Peptide |
Application |
WB |
Bio Background |
Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2. |
Bio References |
Liu F., et al. Proc. Natl. Acad. Sci. U.S.A. 92:10287-10291(1995).O'Neill T.J., et al. J. Biol. Chem. 271:22506-22513(1996).Frantz J.D., et al. J. Biol. Chem. 272:2659-2667(1997).Dong L.Q., et al. J. Biol. Chem. 272:29104-29112(1997).Nantel A., et al. J. Biol. Chem. 273:10475-10484(1998). |
Clonality |
Polyclonal |
Gene ID |
2887 |
Gene Name |
GRB10 |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
GRB10 Antibody (N-term) (Cat. #AP20489a) western blot analysis in A549, MDA-MB453, U251 cell line lysates (35ug/lane).This demonstrates the GRB10 antibody detected the GRB10 protein (arrow). |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Isotype |
Rabbit Ig |
Calculated MW |
67231 |
Antigen Region |
1-30 |
Antigen Source |
Human |
Target/Specificity |
This GRB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GRB10. |
NCBI Accession |
NP_001001549.1; NP_001001550.1; NP_001001555.1; NP_005302.3 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.