Item no. |
ABC-AP1817d-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, IF |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1 |
Available |
|
Primary Accession |
Q676U5 |
Antigen Type |
Recombinant Protein |
Application |
WB, IF |
Bio Background |
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane. |
Bio References |
References for protein:1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) 2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) 3.Greenberg JT. Dev Cell. 8(6):799-801. (2005) 4.Levine B. Cell. 120(2):159-62. (2005) 5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)References for U251 cell line:1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J., Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].3. Geng Y.; Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898]. |
Clonality |
Polyclonal |
Clone/Animal Names |
RB14718 |
Gene ID |
55054 |
Gene Name |
ATG16L1 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 μM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817d ATG16L primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978). |
Type image 1 |
IF |
Dilution image 1 |
1:100 |
Legend image 2 |
Western blot analysis of anti-APG16 Pab (Cat.#AP1817d) in Hela cell line lysates (35ug/lane).APG16(arrow) was detected using the purified Pab. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Isotype |
Rabbit Ig |
Calculated MW |
68265 |
Antigen Source |
HUMAN |
Target/Specificity |
This APG16L antibody is generated from rabbits immunized with a recombinant fragment protein from human APG16L. |
NCBI Accession |
NP_001177195.1; NP_001177196.1; NP_060444.3; NP_110430.5; NP_942593.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.