Item no. |
ABC-AP1814i-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
ATG9A, APG9L1, Autophagy-related protein 9A, APG9-like 1, mATG9 |
Available |
|
Primary Accession |
Q7Z3C6 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). |
Bio References |
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB13941 |
Gene ID |
79065 |
Gene Name |
ATG9A |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-APG9L1 Antibody (S738) at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 94kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG9L1 Antibody (S738) (Cat.#AP1814i), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:10~50 |
Other Accession |
Q5FWU3; Q3T904 |
Predicted |
B, Rat |
Isotype |
Rabbit Ig |
Calculated MW |
94447 |
Antigen Region |
723-753 |
Antigen Source |
HUMAN |
Target/Specificity |
This ATG9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 723~753 amino acids surrounding S738 of human APG9L1. |
NCBI Accession |
NP_001070666.1; NP_076990.4 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.