Item no. |
ABC-AP14937a-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
ALPP, PLAP, Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1 |
Available |
|
Primary Accession |
P05187 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
There are at least four distinct but related alkalinephosphatases: intestinal, placental, placental-like, andliver/bone/kidney (tissue non-specific). The first three arelocated together on chromosome 2 while the tissue non-specific formis located on chromosome 1. The product of this gene is a membranebound glycosylated enzyme, also referred to as the heat stableform, that is expressed primarily in the placenta although it isclosely related to the intestinal form of the enzyme as well as tothe placental-like form. The coding sequence for this form ofalkaline phosphatase is unique in that the 3' untranslated regioncontains multiple copies of an Alu family repeat. In addition, thisgene is polymorphic and three common alleles (type 1, type 2 andtype 3) for this form of alkaline phosphatase have been wellcharacterized. |
Bio References |
Stec, B., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010) :Wang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009)Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009)Zhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009)Roberson, J.R., et al. Pediatr Blood Cancer 51(6):840-842(2008) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB35524 |
Gene ID |
250 |
Gene Name |
ALPP |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
ALPP Antibody (N-term) (Cat. #AP14937a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow). |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of ALPP (arrow) using rabbit polyclonal ALPP Antibody (N-term) (Cat. #AP14937a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPP gene. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
ALPP Antibody (N-term) (AP14937a)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALPP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:10~50 |
Other Accession |
P10696; NP_001623.3 |
Isotype |
Rabbit Ig |
Calculated MW |
57954 |
Antigen Region |
56-83 |
Antigen Source |
HUMAN |
Target/Specificity |
This ALPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the N-terminal region of human ALPP. |
NCBI Accession |
NP_001623.3 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.