Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, FC, IF, IHC-P |
Amount |
80 ul |
Host |
Rabbit |
Item no. |
ABC-AP1470a-ev |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
Q7Z444 |
Antigen Type |
Synthetic Peptide |
Application |
FC, IF, IHC-P, WB |
Bio Background |
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells. |
Bio References |
Kameda, T., Stem Cells 23 (10), 1535-1540 (2005)Takahashi, K., Nature 423 (6939), 541-545 (2003)Miyoshi, J., Nucleic Acids Res. 12 (4), 1821-1828 (1984) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB12690 |
Gene ID |
3266 |
Gene Name |
ERAS |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Immunofluorescence analysis of anti-ERAS Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. |
Type image 1 |
IF |
Dilution image 1 |
1:10~50 |
Legend image 2 |
Western blot analysis of ERAS Antibody (N-term) (Cat.#AP1470a) in K562 cell line lysates (35ug/lane). ERAS (arrow) was detected using the purified Pab. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (A28)(Cat.#AP1470a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50 |
Legend image 4 |
Flow cytometric analysis of hela cells using ERAS Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 4 |
FC |
Dilution image 4 |
1:10~50 |
Isotype |
Rabbit Ig |
Calculated MW |
25287 |
Antigen Region |
13-42 |
Antigen Source |
HUMAN |
Target/Specificity |
This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human ERAS. |
NCBI Accession |
NP_853510.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.