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DPPA4 Antibody (N-term)

DPPA4 Antibody (N-term)

Item no.	ABC-AP1438a-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	DPPA4, Developmental pluripotency-associated protein 4
Available
Primary Accession	Q7L190
Antigen Type	Synthetic Peptide
Application	WB, IHC-P
Bio Background	DppA4 is a member of the Dppa family of developmental pluripotency associated transcription factors. Dppa4 is a target of Oct4, Sox2 and Nanog. It may play a role in maintaining cell pluripotentiality.
Bio References	Bortvin, A., Development 130 (8), 1673-1680 (2003
Clonality	Polyclonal
Clone/Animal Names	RB13363
Gene ID	55211
Gene Name	DPPA4
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-DPPA4 Antibody (N-term) at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Western blot analysis of DPPA4 Antibody (N-term) (Cat. #AP1438a) in CEM cell line lysates (35ug/lane). DPPA4 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	AP1438a staining DPPA4 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:25
Isotype	Rabbit Ig
Calculated MW	33541
Antigen Region	50-79
Antigen Source	HUMAN
Target/Specificity	This DPPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the N-terminal region of human DPPA4.
NCBI Accession	NP_060659.3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 80 ul

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Delivery expected until 10/4/2024

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