ADD3 Antibody (C-term)

Synthetic Peptide

Adducins are heteromeric proteins composed of different
subunits referred to as adducin alpha, beta and gamma. The three
subunits are encoded by distinct genes and belong to a family of
membrane skeletal proteins involved in the assembly of
spectrin-actin network in erythrocytes and at sites of cell-cell
contact in epithelial tissues. While adducins alpha and gamma are
ubiquitously expressed, the expression of adducin beta is
restricted to brain and hematopoietic tissues. Adducin, originally
purified from human erythrocytes, was found to be a heterodimer of
adducins alpha and beta. Polymorphisms resulting in amino acid
substitutions in these two subunits have been associated with the
regulation of blood pressure in an animal model of hypertension.
Heterodimers consisting of alpha and gamma subunits have also been
described. Structurally, each subunit is comprised of two distinct
domains. The amino-terminal region is protease resistant and
globular in shape, while the carboxy-terminal region is protease
sensitive. The latter contains multiple phosphorylation sites for
protein kinase C, the binding site for calmodulin, and is required
for association with spectrin and actin. Alternatively spliced
adducin gamma transcripts encoding different isoforms have been
described. The functions of the different isoforms are not known.

Polyclonal

120

Affinity Purified Rabbit Polyclonal Antibody (Pab)

ADD3 Antibody (C-term) (Cat. #AP14227b) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ADD3 antibody detected the ADD3 protein (arrow).

1:1000

Rabbit Ig

552-580

This ADD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 552-580 amino acids from the C-terminal region of human ADD3.