Item no. |
ABC-AP14227B |
Manufacturer |
Abcepta
|
Amount |
400 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
ADD3, ADDL, Gamma-adducin, Adducin-like protein 70 |
Available |
|
Primary Accession |
Q9UEY8 |
Antigen Type |
Synthetic Peptide |
Application |
WB |
Bio Background |
Adducins are heteromeric proteins composed of differentsubunits referred to as adducin alpha, beta and gamma. The threesubunits are encoded by distinct genes and belong to a family ofmembrane skeletal proteins involved in the assembly ofspectrin-actin network in erythrocytes and at sites of cell-cellcontact in epithelial tissues. While adducins alpha and gamma areubiquitously expressed, the expression of adducin beta isrestricted to brain and hematopoietic tissues. Adducin, originallypurified from human erythrocytes, was found to be a heterodimer ofadducins alpha and beta. Polymorphisms resulting in amino acidsubstitutions in these two subunits have been associated with theregulation of blood pressure in an animal model of hypertension.Heterodimers consisting of alpha and gamma subunits have also beendescribed. Structurally, each subunit is comprised of two distinctdomains. The amino-terminal region is protease resistant andglobular in shape, while the carboxy-terminal region is proteasesensitive. The latter contains multiple phosphorylation sites forprotein kinase C, the binding site for calmodulin, and is requiredfor association with spectrin and actin. Alternatively splicedadducin gamma transcripts encoding different isoforms have beendescribed. The functions of the different isoforms are not known. |
Bio References |
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)Garcia-Barcelo, M.M., et al. Hum. Mol. Genet. 19(14):2917-2925(2010)Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)Seidlerova, J., et al. Am. J. Hypertens. 22(1):21-26(2009) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB34232 |
Gene ID |
120 |
Gene Name |
ADD3 |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
ADD3 Antibody (C-term) (Cat. #AP14227b) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ADD3 antibody detected the ADD3 protein (arrow). |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Other Accession |
NP_001112.2; NP_063968.1 |
Isotype |
Rabbit Ig |
Calculated MW |
79155 |
Antigen Region |
552-580 |
Antigen Source |
HUMAN |
Target/Specificity |
This ADD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 552-580 amino acids from the C-terminal region of human ADD3. |
NCBI Accession |
NP_001112.2; NP_058432.1; NP_063968.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.