« Back
Home /
PRMT5 Antibody (N-term)

PRMT5 Antibody (N-term)

Item no.	ABC-AP13773a-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PRMT5, N-terminally processed, HRMT1L5, IBP72, JBP1, SKB1, Protein arginine N-methyltransferase 5, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Shk1 kinase-binding protein 1 homolog, Protein arginine N-methyltransferase 5, SKB1Hs, SKB1 homolog, 211-
Available
Primary Accession	O14744
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
Bio References	Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010) Rank, G., et al. Blood 116(9):1585-1592(2010) Cesaro, E., et al. J. Biol. Chem. 284(47):32321-32330(2009) Zhao, Q., et al. Nat. Struct. Mol. Biol. 16(3):304-311(2009) Bruns, A.F., et al. Biol. Chem. 390(1):59-65(2009)
Clonality	Polyclonal
Clone/Animal Names	RB33884
Gene ID	10419
Gene Name	PRMT5
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-PRMT5 Antibody (N-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	PRMT5 Antibody (N-term) (Cat. #AP13773a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:10~50
Other Accession	Q4R5M3; A7YW45; NP_006100.2; NP_001034708.1
Predicted	B, Pr
Isotype	Rabbit Ig
Calculated MW	72684
Antigen Region	76-104
Antigen Source	HUMAN
Target/Specificity	This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the N-terminal region of human PRMT5.
NCBI Accession	NP_001034708.1; NP_006100.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-AP13773a-ev-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 80 ul

available

Delivery expected until 10/4/2024