Item no. |
ABC-AP13773a-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
PRMT5, N-terminally processed, HRMT1L5, IBP72, JBP1, SKB1, Protein arginine N-methyltransferase 5, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Shk1 kinase-binding protein 1 homolog, Protein arginine N-methyltransferase 5, SKB1Hs, SKB1 homolog, 211- |
Available |
|
Primary Accession |
O14744 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. |
Bio References |
Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010)Rank, G., et al. Blood 116(9):1585-1592(2010)Cesaro, E., et al. J. Biol. Chem. 284(47):32321-32330(2009)Zhao, Q., et al. Nat. Struct. Mol. Biol. 16(3):304-311(2009)Bruns, A.F., et al. Biol. Chem. 390(1):59-65(2009) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB33884 |
Gene ID |
10419 |
Gene Name |
PRMT5 |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-PRMT5 Antibody (N-term) at 1:1000 dilutionLane 1: Jurkat whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
PRMT5 Antibody (N-term) (Cat. #AP13773a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:10~50 |
Other Accession |
Q4R5M3; A7YW45; NP_006100.2; NP_001034708.1 |
Predicted |
B, Pr |
Isotype |
Rabbit Ig |
Calculated MW |
72684 |
Antigen Region |
76-104 |
Antigen Source |
HUMAN |
Target/Specificity |
This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the N-terminal region of human PRMT5. |
NCBI Accession |
NP_001034708.1; NP_006100.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.