Item no. |
ABC-AP13305b-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
ERO1L, ERO1-like protein alpha, Endoplasmic oxidoreductin-1-like protein, Oxidoreductin-1-L-alpha, ERO1-L, ERO1-L-alpha, 184- |
Available |
|
Primary Accession |
Q96HE7 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. |
Bio References |
Inaba, K., et al. EMBO J. 29(19):3330-3343(2010)Appenzeller-Herzog, C., et al. EMBO J. 29(19):3318-3329(2010)Swiatkowska, M., et al. J. Biol. Chem. 285(39):29874-29883(2010)Chambers, J.E., et al. J. Biol. Chem. 285(38):29200-29207(2010)Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
Clonality |
Polyclonal |
Clone/Animal Names |
RB33290 |
Gene ID |
30001 |
Gene Name |
|
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
ERO1L Antibody (C-term) (Cat. #AP13305b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ERO1L antibody detected the ERO1L protein (arrow). |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
ERO1L Antibody (C-term) (Cat. #AP13305b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ERO1L Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:10~50 |
Other Accession |
NP_055399.1 |
Isotype |
Rabbit Ig |
Calculated MW |
54393 |
Antigen Region |
350-379 |
Antigen Source |
HUMAN |
Target/Specificity |
This ERO1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-379 amino acids from the C-terminal region of human ERO1L. |
NCBI Accession |
NP_055399.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.