Item no. |
ABC-AP1317a |
Manufacturer |
Abcepta
|
Amount |
400 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, FC, IF, IHC-P |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
PUMA, BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis |
Available |
|
Primary Accession |
Q9BXH1 |
Antigen Type |
Synthetic Peptide |
Application |
FC, IF, IHC-P, WB |
Bio Background |
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. |
Bio References |
Liu, F.T., et al., Biochem. Biophys. Res. Commun. 310(3):956-962 (2003). Hoque, M.O., et al., Cancer Lett. 199(1):75-81 (2003). Yu, J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(4):1931-1936 (2003). Han, J., et al., Proc. Natl. Acad. Sci. U.S.A. 98(20):11318-11323 (2001). Nakano, K., et al., Mol. Cell 7(3):683-694 (2001). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB01353 |
Gene ID |
27113 |
Gene Name |
BBC3 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Confocal immunofluorescent analysis of Puma BH3 Domain Antibody(Cat#AP1317a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Type image 1 |
IF |
Dilution image 1 |
1:10~50 |
Legend image 2 |
Western blot analysis of anti-Puma BH3 domain Pab (Cat. #AP1317a) in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody (Cat.#AP1317a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:10~50 |
Legend image 4 |
Puma BH3 Domain Antibody (Cat. #AP1317a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 4 |
FC |
Dilution image 4 |
1:10~50 |
Other Accession |
Q80ZG6; Q99ML1 |
Predicted |
M, Rat |
Isotype |
Rabbit Ig |
Calculated MW |
20532 |
Antigen Region |
119-154 |
Antigen Source |
HUMAN |
Target/Specificity |
This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain. |
NCBI Accession |
NP_001120712.1; NP_001120713.1; NP_001120714.1; NP_055232.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.