Item no. |
ABC-AP11110c |
Manufacturer |
Abcepta
|
Amount |
400 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC, IHC-P |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CAD, CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, Aspartate carbamoyltransferase, Dihydroorotase |
Available |
|
Primary Accession |
P27708 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P, FC |
Bio Background |
The de novo synthesis of pyrimidine nucleotides isrequired for mammalian cells to proliferate. This gene encodes atrifunctional protein which is associated with the enzymaticactivities of the first 3 enzymes in the 6-step pathway ofpyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein isregulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPKcascade and de novo biosynthesis of pyrimidine nucleotides. |
Bio References |
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Ahuja, V., et al. J. Inherit. Metab. Dis. 31(4):481-491(2008)Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)Olsen, J.V., et al. Cell 127(3):635-648(2006) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB18384 |
Gene ID |
790 |
Gene Name |
|
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-CAD Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLane 3: SW620 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 243 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Legend image 2 |
Anti-CAD Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 243 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:2000 |
Legend image 3 |
All lanes : Anti-CAD Antibody (Center) at 1:2000 dilutionLane 1: Jurkat whole cell lysateLane 2: 293T/17 whole cell lysateLane 3: Hela whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 243 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 3 |
WB |
Dilution image 3 |
1:2000 |
Legend image 4 |
CAD Antibody (Center) (Cat. #AP11110c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CAD antibody detected the CAD protein (arrow). |
Type image 4 |
WB |
Dilution image 4 |
1:1000 |
Legend image 5 |
CAD Antibody (Center) (Cat. #AP11110c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 5 |
IHC-P |
Dilution image 5 |
1:10~50 |
Legend image 6 |
AP11110c staining CAD in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
Type image 6 |
IHC-P |
Dilution image 6 |
1:25 |
Legend image 7 |
Overlay histogram showing Hela cells stained with AP11110c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11110c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Type image 7 |
FC |
Dilution image 7 |
1:25 |
Other Accession |
B2RQC6; NP_004332.2 |
Predicted |
M |
Isotype |
Rabbit Ig |
Calculated MW |
242984 |
Antigen Region |
780-809 |
Antigen Source |
HUMAN |
Target/Specificity |
This CAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 780-809 amino acids from the Central region of human CAD. |
NCBI Accession |
NP_004332.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.