Item no. |
ABC-AP11075c |
Manufacturer |
Abcepta
|
Amount |
400 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC, IF, IHC-P |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
NR0B2, SHP, Nuclear receptor subfamily 0 group B member 2, Orphan nuclear receptor SHP, Small heterodimer partner |
Available |
|
Primary Accession |
Q15466 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P, IF, FC |
Bio Background |
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. |
Bio References |
Zhou, T., et al. J. Biol. Chem. 285(32):24871-24881(2010) Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010) Kim, K., et al. Biochem. J. 427(3):413-422(2010) Yang, Z., et al. J. Int. Med. Res. 38(1):142-149(2010) Ondracek, C.R., et al. J. Virol. 83(23):12535-12544(2009) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB29166 |
Gene ID |
8431 |
Gene Name |
NR0B2 |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Confocal immunofluorescent analysis of NR0B2 Antibody (Center)(Cat#AP11075c) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Type image 1 |
IF |
Dilution image 1 |
1:10~50 |
Legend image 2 |
NR0B2 Antibody (Center)(PEI 1:100) (Cat. #AP11075c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR0B2 antibody detected the NR0B2 protein (arrow). |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
NR0B2 antibody (Center) (Cat. #AP11075c) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR0B2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Legend image 4 |
NR0B2 Antibody (Center) (Cat. #AP11075c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 4 |
FC |
Dilution image 4 |
1:10~50 |
Other Accession |
NP_068804.1 |
Isotype |
Rabbit Ig |
Calculated MW |
28058 |
Antigen Region |
56-83 |
Antigen Source |
HUMAN |
Target/Specificity |
This NR0B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the Central region of human NR0B2. |
NCBI Accession |
NP_068804.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.