« Back
Home /
PEMT Antibody (C-term)

PEMT Antibody (C-term)

Item no.	ABC-AP1025B-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PNMT, PEMT, PEMPT, Phosphatidylethanolamine N-methyltransferase, PEMT2, PEAMT
Available
Primary Accession	Q9UBM1
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	PEMT is an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17.
Bio References	Walkey C.J., Biochim. Biophys. Acta 1436:405-412(1999). Shields D.J., Biochim. Biophys. Acta 1532:105-114(2001). Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Clonality	Polyclonal
Clone/Animal Names	RB12628
Gene ID	10400
Gene Name	PEMT {ECO:0000255\|HAMAP-Rule:MF_03216}
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-PEMT Antibody (C-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: Mouse liver tissue lysate Lane 4: Mouse ovary tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Immunohistochemical analysis of AP1025B on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 2	IHC-P
Dilution image 2	1:100
Legend image 3	Immunohistochemical analysis of AP1025B on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:100
Isotype	Rabbit Ig
Calculated MW	22134
Antigen Region	170-199
Antigen Source	HUMAN
Target/Specificity	This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-199 amino acids from the C-terminal region of human PEMT.
NCBI Accession	NP_001254480.1; NP_001254481.1; NP_009100.2; NP_680477.1; NP_680478.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-AP1025B-ev-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 80 ul

available

Delivery expected until 11/8/2024

Express Delivery: Save 28 days

Compare

Add to wishlist

Recommend by mail