Item no. |
ABC-AO2316a |
Manufacturer |
Abcepta
|
Amount |
100 ul |
Category |
|
Type |
Antibody Primary |
Applications |
WB, FC, IHC, ELISA |
Clone |
3H6H2 |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
mitochondrial, MRPL42, MRPL31, MRP-L31, MRPS32, RPML31, 39S ribosomal protein L42, 28S ribosomal protein S32, 39S ribosomal protein L31, L31mt, L42mt, MRP-L42, MRP-S32, S32mt |
Available |
|
Primary Accession |
Q9Y6G3 |
Application |
WB, IHC, FC, E |
Bio References |
1. Genomics. 2003 May; 81(5):468-80. 2. J Biol Chem. 2001 Nov 23; 276(47):43958-69. |
Clonality |
Monoclonal |
Gene ID |
28977 |
Gene Name |
MRPL42 |
Subtitle |
Purified Mouse Monoclonal Antibody |
Reactivity |
H |
Legend image 1 |
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng); |
Type image 1 |
E |
Dilution image 1 |
1/10000 |
Legend image 2 |
Figure 1: Western blot analysis using MRPL42 mAb against human MRPL42 recombinant protein. (Expected MW is 41.2 kDa) |
Type image 2 |
WB |
Dilution image 2 |
1/500 - 1/2000 |
Legend image 3 |
Figure 2: Western blot analysis using MRPL42 mAb against HEK293 (1) and MRPL42 (AA: 10-142)-hIgGFc transfected HEK293 (2) cell lysate. |
Type image 3 |
WB |
Dilution image 3 |
1/500 - 1/2000 |
Legend image 4 |
Figure 3: Western blot analysis using MRPL42 mouse mAb against HL7702 (1), SMMC-7721 (2), HEK293 (3) , HeLa (4) and Raji (5) cell lysate. |
Type image 4 |
WB |
Dilution image 4 |
1/500 - 1/2000 |
Legend image 5 |
Figure 4: Flow cytometric analysis of HepG2 cells using MRPL42 mouse mAb (green) and negative control (purple). |
Type image 5 |
FC |
Dilution image 5 |
1/200 - 1/400 |
Legend image 6 |
Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using MRPL42 mouse mAb with DAB staining. |
Type image 6 |
IHC |
Dilution image 6 |
1/200 - 1/1000 |
Legend image 7 |
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MRPL42 mouse mAb with DAB staining. |
Type image 7 |
IHC |
Dilution image 7 |
1/200 - 1/1000 |
Isotype |
IgG1 |
Calculated MW |
16661 |
Formulation |
Purified antibody in PBS with 0.05% sodium azide |
Description |
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. |
|
Clone: 3H6H2 |
Immunogen |
Purified recombinant fragment of human MRPL42 (AA: 10-142) expressed in E. Coli. |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.