« Back
Home /
GLI1 Antibody

GLI1 Antibody

Item no.	ABC-AO2089a
Manufacturer	Abcepta
Amount	100 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, FC, IHC, ICC, ELISA
Clone	1B9F8
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	GLI1, GLI, Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI
Available
Primary Accession	P08151
Application	WB, IHC, FC, ICC, E
Bio References	1.Mol Cancer. 2014 Jun 3; 13:137.2.J Hematol Oncol. 2014 Mar 30; 7:28.
Clonality	Monoclonal
Gene ID	2735
Gene Name	GLI1
Subtitle	Purified Mouse Monoclonal Antibody
Reactivity	H
Legend image 1	Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Type image 1	E
Dilution image 1	1/10000
Legend image 2	Figure 2:Western blot analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)
Type image 2	WB
Dilution image 2	1/500 - 1/2000
Legend image 3	Figure 3:Western blot analysis using GLI1 mAb against HEK293 (1) and GLI1 (AA: 284-449)-hIgGFc transfected HEK293 (2) cell lysate.
Type image 3	WB
Dilution image 3	1/500 - 1/2000
Legend image 4	Figure 4:Immunofluorescence analysis of Hela cells using GLI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Type image 4	IF
Dilution image 4	1/200 - 1/1000
Legend image 5	Figure 5:Flow cytometric analysis of HepG2 cells using GLI1 mouse mAb (green) and negative control (red).
Type image 5	FC
Dilution image 5	1/200 - 1/400
Legend image 6	Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using GLI1 mouse mAb with DAB staining.
Type image 6	IHC
Dilution image 6	1/200 - 1/1000
Legend image 7	Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GLI1 mouse mAb with DAB staining.
Type image 7	IHC
Dilution image 7	1/200 - 1/1000
Isotype	IgG1
Calculated MW	117904
Formulation	Purified antibody in PBS with 0.05% sodium azide
Description	This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
	Clone: 1B9F8
Immunogen	Purified recombinant fragment of human GLI1 (AA: 284-449) expressed in E. Coli.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-AO2089a-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 100 ul

available

Delivery expected until 9/6/2024

Express Delivery: Save 7 days

Compare

Add to wishlist

Recommend by mail