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PLCG2 Antibody

PLCG2 Antibody

Item no.	ABC-AO2065a
Manufacturer	Abcepta
Amount	100 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, FC, ICC, ELISA
Clone	2D9E8
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	1-phosphatidylinositol 4, PLCG2, 5-bisphosphate phosphodiesterase gamma-2, PLC-IV, PLC-gamma-2, Phospholipase C-gamma-2, Phospholipase C-IV, Phosphoinositide phospholipase C-gamma-2, 3.1.4.11
Available
Primary Accession	P16885
Application	WB, FC, ICC, E
Bio References	1.J Biol Chem. 2005 Nov 25; 280(47):38923-31.2.World J Gastroenterol. 2003 Nov; 9(11):2413-8.
Clonality	Monoclonal
Gene ID	5336
Gene Name	PLCG2 (HGNC:9066)
Subtitle	Purified Mouse Monoclonal Antibody
Reactivity	H
Legend image 1	Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Type image 1	E
Dilution image 1	1/10000
Legend image 2	Figure 2:Western blot analysis using PLCG2 mAb against human PLCG2 (AA: 826-985) recombinant protein. (Expected MW is 44.6 kDa)
Type image 2	WB
Dilution image 2	1/500 - 1/2000
Legend image 3	Figure 3:Western blot analysis using PLCG2 mAb against HEK293 (1) and PLCG2 (AA: 826-985)-hIgGFc transfected HEK293 (2) cell lysate.
Type image 3	WB
Dilution image 3	1/500 - 1/2000
Legend image 4	Figure 4:Immunofluorescence analysis of MCF-7 cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Type image 4	IF
Dilution image 4	1/200 - 1/1000
Legend image 5	Figure 5:Flow cytometric analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).
Type image 5	FC
Dilution image 5	1/200 - 1/400
Isotype	IgG1
Calculated MW	147870
Formulation	Purified antibody in PBS with 0.05% sodium azide
Description	The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4, 5-bisphosphate to 1D-myo-inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
	Clone: 2D9E8
Immunogen	Purified recombinant fragment of human PLCG2 (AA: 826-985) expressed in E. Coli.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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