WT1 Antibody

Recombinant Protein

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.

Monoclonal

WT1

H

E

Figure 1: Western blot analysis using WT1 mAb against human WT1 (AA: 314-479) recombinant protein. (Expected MW is 47.6 kDa)

1/500 - 1/2000

WB

Figure 3: Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).

1/200 - 1/400

49188

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Purified recombinant fragment of human WT1 (AA: 314-479) expressed in E. Coli.