Item no. |
ABC-AO1868a |
Manufacturer |
Abcepta
|
Amount |
100 ul |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IHC, ELISA |
Clone |
10B11C8 |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
MAPK14, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, CSBP, CSBP1, CSBP2, CSPB1, SAPK2A, MAX-interacting protein 2, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAP kinase p38 alpha, Stress-activated protein kinase 2a, MXI2, SAPK2a, 2.7.11.24 |
Available |
|
Primary Accession |
Q16539 |
Antigen Type |
Recombinant Protein |
Application |
WB, IHC, E |
Bio Background |
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. ; |
Bio References |
1. Cytokine. 2012 Oct; 60(1):114-21. 2. Clin Cancer Res. 2012 Aug 1; 18(15):4037-47. |
Clonality |
Monoclonal |
Gene ID |
1432 |
Gene Name |
MAPK14 |
Subtitle |
Purified Mouse Monoclonal Antibody |
Reactivity |
H, M, Rat, Pr |
Legend image 1 |
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng); |
Type image 1 |
E |
Dilution image 1 |
1/10000 |
Legend image 2 |
Figure 1: Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 32.6 kDa) |
Type image 2 |
WB |
Dilution image 2 |
1/500 - 1/2000 |
Legend image 3 |
Figure 2: Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate. |
Type image 3 |
WB |
Dilution image 3 |
1/500 - 1/2000 |
Legend image 4 |
Figure 3: Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate. |
Type image 4 |
WB |
Dilution image 4 |
1/500 - 1/2000 |
Legend image 5 |
Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using MAPK14 mouse mAb with DAB staining. |
Type image 5 |
IHC |
Dilution image 5 |
1/200 - 1/1000 |
Isotype |
IgG1 |
Calculated MW |
41293 |
Formulation |
Purified antibody in PBS with 0.05% sodium azide |
Description |
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
|
Clone: 10B11C8 |
Immunogen |
Purified recombinant fragment of human MAPK14 (AA: 299-360) expressed in E. Coli. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.