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CD22 Antibody

CD22 Antibody

Item no.	ABC-AO1851a
Manufacturer	Abcepta
Amount	100 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, FC, ICC, ELISA
Clone	1A3A11
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	CD22, SIGLEC2, Siglec-2, BL-CAM, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14
Available
Primary Accession	P20273
Antigen Type	Recombinant Protein
Application	WB, FC, ICC, E
Bio Background	CD22 may be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules ;
Bio References	1. Cancer Res. 2012 Nov 1; 72(21):5556-65. 2. J Innate Immun. 2011; 3(4):411-9.
Clonality	Monoclonal
Gene ID	933
Gene Name	CD22
Subtitle	Purified Mouse Monoclonal Antibody
Reactivity	H
Legend image 1	Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Type image 1	E
Dilution image 1	1/10000
Legend image 2	Figure 1: Western blot analysis using CD22 mAb against human CD22 recombinant protein. (Expected MW is 37 kDa)
Type image 2	WB
Dilution image 2	1/500 - 1/2000
Legend image 3	Figure 2: Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.
Type image 3	WB
Dilution image 3	1/500 - 1/2000
Legend image 4	Figure 3: Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Type image 4	IF
Dilution image 4	1/200 - 1/1000
Legend image 5	Figure 4: Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).
Type image 5	FC
Dilution image 5	1/200 - 1/400
Isotype	IgG1
Calculated MW	95348
Formulation	Purified antibody in PBS with 0.05% sodium azide
Description	CD22 may be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules
	Clone: 1A3A11
Immunogen	Purified recombinant fragment of human CD22 (AA: 621-725) expressed in E. Coli.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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