Item no. |
ABC-AO1765a |
Manufacturer |
Abcepta
|
Amount |
100 ul |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IHC, ICC, ELISA |
Clone |
4E7 |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
HAS2, HA synthase 2, Hyaluronan synthase 2, Hyaluronate synthase 2, Hyaluronic acid synthase 2, 2.4.1.212 |
Available |
|
Primary Accession |
Q92819 |
Antigen Type |
Recombinant Protein |
Application |
WB, IHC, ICC, E |
Bio References |
1.Cancer Res. 2012 Jan 15; 72(2):537-47.2.J Biol Chem. 2011 Sep 23; 286(38):33632-40. |
Clonality |
Monoclonal |
Gene ID |
3037 |
Gene Name |
HAS2 |
Subtitle |
Purified Mouse Monoclonal Antibody |
Reactivity |
H |
Legend image 1 |
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng); |
Type image 1 |
E |
Dilution image 1 |
1/10000 |
Legend image 2 |
Figure 1: Western blot analysis using HAS2 mAb against human HAS2 recombinant protein. (Expected MW is 37.5 kDa) |
Type image 2 |
WB |
Dilution image 2 |
1/500 - 1/2000 |
Legend image 3 |
Figure 2: Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate. |
Type image 3 |
WB |
Dilution image 3 |
1/500 - 1/2000 |
Legend image 4 |
Figure 3: Immunofluorescence analysis of HeLa cells using HAS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. |
Type image 4 |
IF |
Dilution image 4 |
1/100 - 1/500 |
Legend image 5 |
Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using HAS2 mouse mAb with DAB staining. |
Type image 5 |
IHC |
Dilution image 5 |
1/200 - 1/1000 |
Isotype |
IgG1 |
Calculated MW |
63566 |
Formulation |
Purified antibody in PBS with 0.05% sodium azide |
Description |
Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1. |
|
Clone: 4E7 |
Immunogen |
Purified recombinant fragment of human HAS2 (AA: 67-170) expressed in E. Coli. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.