NPT Antibody

Recombinant Protein

1. Landis WG et.al Human and ecological Risk Assessment 6(5): 875-899. 2. Stewart CN et.al BioTechniques 29(4): 832-843. 3. Thomson JA et.al Journal of Food Science 66(2): 188-193.

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using NPT mouse mAb against truncated NPT recombinant protein.

1/500 - 1/2000

IHC

IgG2a

Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Purified recombinant fragment of NPT expressed in E. Coli.