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Phospho-Ser19 Tryptophan Hydroxylase 2 Antibody

Phospho-Ser19 Tryptophan Hydroxylase 2 Antibody

Item no.	ABC-AN1134
Manufacturer	Abcepta
Amount	100 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Applications	WB
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Tph2, Neuronal tryptophan hydroxylase, Tryptophan 5-hydroxylase 2, Tryptophan 5-monooxygenase 2, Ntph
Available
Primary Accession	Q8CGU9
Application	WB
Bio Background	Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).
Bio References	Jiang GC, Yohrling GJ, Schmitt JD, Vrana KE (2000) Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. J Mol Biol 302:1005-1017. Johansen PA, Jennings I, Cotton RG, Kuhn DM (1996) Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J Neurochem 66:817-823. Martinez A, Knappskog PM, Haavik J (2001) Structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091. McKinney J, Knappskog PM, Haavik J (2005) Different properties of the central and peripheral forms of human tryptophan hydrpxylase. J Neurochem 92(2):311-20. Kuhn DM, Sakowski SA, Geddes TJ, Wilkerson C, Haycock JW (2007) Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine-19 as the substrate site for calcium, calmodulin-dependent protein kinase II. J Neurochem 103(4):1567-73.
Clonality	polyclonal
Gene ID	317675
Gene Name	Tph2
Subtitle	Affinity purified rabbit polyclonal antibody
Reactivity	Rat
Legend image 1	Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) showing specific immunolabeling of the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.
Type image 1	WB
Dilution image 1	1:1000
Predicted	B, M, Zf
Calculated MW	55621
Antigen Source	rat
Target/Specificity	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 conjugated to KLH.
Antibody Specificity	Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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