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NMDA Receptor, NR1 Subunit Antibody

NMDA Receptor, NR1 Subunit Antibody

Item no.	ABC-AN1050
Manufacturer	Abcepta
Amount	15 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Culture supernatant
Applications	WB
Clone	R1JHL
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Glutamate receptor ionotropic, Grin1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, Nmdar1, GluN1, NMDA 1
Available
Primary Accession	P35439
Application	WB
Bio Background	The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and ?-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1-subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Bio References	Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025. Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577. Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33. Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724. Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neurosci 2:331-338. Snell LD, Nunley KR, Lickteig RL, Browning MD, Tabakoff B, Hoffman PL (1996) Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Mol Brain Res 40:71-78. Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358. Li JH, Wang YH, Wolfe BB, Krueger KE, Corsi L, Stocca G, Vicini S (1998) Developmental changes in localization of NMDA receptor subunits in primary cultures of cortical neurons. Eur J Neurosci. 10(5):1704-15. Kurtis D. Davies, Susan M. Goebel-Goody, Steven J. Coultrap, and Michael D. Browning (2008) Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4- isoxazolepropionic Acid (AMPA) Receptor Internalization J. Biol. Chem., 283: 33138 - 33146. Guohua Zhang, Yuanlin Dong, Bin Zhang, Fumito Ichinose, Xu Wu, Deborah J. Culley, Gregory Crosby, Rudolph E. Tanzi, and Zhongcong Xie (2008) Isoflurane-Induced Caspase-3 Activation Is Dependent on Cytosolic Calcium and Can Be Attenuated by Memantine J. Neurosci., 4551 - 4560. Tianna R. Hicklin, Peter H. Wu, Richard A. Radcliffe, Ronald K. Freund, Susan M. Goebel-Goody, Paulo R. Correa, William R. Proctor, Paul J. Lombroso, and Michael D. Browning (2011) Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase PNAS, Apr 2011; 108: 6650 - 6655. Note: Dr. Michael Browning, a co-author of four of the cited papers, is President and founder of PhosphoSolutions. Page 2
Clonality	monoclonal
Gene ID	24408
Gene Name	Grin1
Subtitle	Mouse monoclonal antibody
Reactivity	M, Rat
Legend image 1	Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor.
Type image 1	WB
Dilution image 1	1:1000
Calculated MW	105509
Antigen Source	rat
Target/Specificity	Fusion protein containing amino acids 1-564 of the NR1 subunit.
	Clone: R1JHL
Antibody Specificity	Specific for the ~120k NR1 subunit of the NMDA receptor.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 15 ug

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