CD276 Antibody

Synthesized Peptide

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

monoclonal

80381

Purified Mouse Monoclonal Antibody (Mab)

All lanes : Anti-CD276 Antibody at 1:4000 dilution
Lane 1: 293T-17 whole cell lysate
Lane 2: A-431 whole cell lysate
Lane 3: U-20S whole cell lysate
Lane 4: LNCap whole cell lysate
Lane 5: Hela whole cell lysate
Lane 6: MCF-7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 57 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:4000

IHC

Immunohistochemical analysis of CD276 Antibody in 293T(untransfected) cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

1:500

IHC-P

H

57235

This CD276 antibody is generated from a mouse immunized with a recombinant protein from the human region of human CD276.