Item no. |
ABC-AM8677b |
Manufacturer |
Abcepta
|
Amount |
200 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Mouse |
Isotype |
IgG1 kappa |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CD276, B7H3, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, Costimulatory molecule, B7-H3 |
Available |
|
Primary Accession |
Q5ZPR3 |
Antigen Type |
Synthesized Peptide |
Application |
WB, IHC-P |
Bio Background |
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. |
Bio References |
Chapoval A.I., et al.Nat. Immunol. 2:269-274(2001).Steinberger P., et al.J. Immunol. 172:2352-2359(2004).Clark H.F., et al.Genome Res. 13:2265-2270(2003).Ota T., et al.Nat. Genet. 36:40-45(2004).Otsuki T., et al.DNA Res. 12:117-126(2005). |
Clonality |
monoclonal |
Clone/Animal Names |
2009CT652.35.9 |
Gene ID |
80381 |
Gene Name |
CD276 |
Subtitle |
Purified Mouse Monoclonal Antibody (Mab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-CD276 Antibody at 1:4000 dilutionLane 1: 293T-17 whole cell lysateLane 2: A-431 whole cell lysateLane 3: U-20S whole cell lysateLane 4: LNCap whole cell lysateLane 5: Hela whole cell lysateLane 6: MCF-7 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:4000 |
Legend image 2 |
Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1(Cat#AM8677B). AM8677B was diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. |
Type image 2 |
IHC |
Dilution image 2 |
1:400 |
Legend image 3 |
Immunohistochemical analysis of CD276 Antibody in 293T(untransfected) cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:500 |
Legend image 4 |
Immunohistochemical analysis of CD276 Antibody in MCF-7 cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
Type image 4 |
IHC-P |
Dilution image 4 |
1:25 |
Predicted |
H |
Isotype |
IgG1, κ |
Calculated MW |
57235 |
Antigen Source |
HUMAN |
Target/Specificity |
This CD276 antibody is generated from a mouse immunized with a recombinant protein from the human region of human CD276. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.